Maize is the most important agricultural crop in Mexico is planted throughout the country, is the staple food of the people and the image of the cornfield, is a symbol of mexican culture. Physical and physiological quality of maize, are of the utmost importance for producers and farmers to estimate the efficiency of the seeds that will lead to their production. In this work, the physical, physiological and biochemical quality of maize seed was studied, 13 genotypes Genetic Improvement Program of Instituto Tecnológico de Roque (ITR) of Celaya, Guanajuato were used, 2 collects of the State of Hidalgo, the race Jala and 6 commercial hybrids. We evaluated: thousand seed weight, hectoliter weight, seed germination, accelerated aging and plumule length. The hectoliter weight Roque 0, Roque 1, Roque 2, Amarillo, Amarillo 2 and the hybrid CRM-52 were above of 75 kg/hL; the greater weight of 1000 seeds correspond to the Jala race (499 g), Roque, SB- 309, Roque 2, 12013, 42013, showed 100 % of germination, however all materials were in the values established by certification standards that is at least 85 %. In the accelerated aging test highlighted SB-39, 22013 Roque and Roque 1 with 99 %. The greater length of plumule Roque obtained with an emergency length of 11.25 cm followed by Roque 2 with 10.7 cm. For biochemical characterization, concentration was assessed and total soluble protein content: albumins, globulins, prolamins and glutelin. Genotypes 12013, 42013 with the highest levels in albumins (g protein per 100 g of flour): 1.34 and 1.35 in the globulin fraction, Amarillo and B2013 with 0.71 and 0.95 in the prolamins fraction, Amarillo 1 and 2 with 2.38 and 1.64, followed by Roque with 1.54 and for the glutelin fraction, Amarillo 2 and Roque 2 were the cultivars with the highest concentration (0.71 and 0.62).

Keywords: Protein, seed physic quality, seed physiological quality, solubility.